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alexa fluor 488 molecular weight

alexa fluor 488 molecular weight

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). Alexa Fluor dyes are frequently used as cell and tissue labels in fluorescence microscopy and cell biology. Use Alexa Fluor ® 488 conjugated to secondary antibodies to get bright and sensitive immunofluorescent staining in cell and tissue fluorescent microscopy. For research use only. AFDye™ 488 NHS Ester (Alexa Fluor® 488 NHS Ester equivalent) is an amine reactive, green-emitting dye routinely used to label proteins or antibodies through primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. Alexa Fluor® 488-conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. Thus the receptor’s predicted molecular weight of 93 kDa is increased to a native molecular weight of 120-160 kDa (3, 4). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company. Labeled antibodies are typically separated from free Alexa Fluor™ dye using a gel filtration column, such as Sephadex™ G-25, BioGel™ P-30, or equivalent. Alexa Fluor™ 488 is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line. Cowan NJ, et al. 1 Product Result Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587. The average molecular weight is reported to be about 160 kD. Learn more about the: Not for resale. It is recommended that the reagent be titrated for optimal performance for each application. The brightest and most photostable green dye for sensitive detection in live or fixed cells; commonly used with Alexa Fluor 350, 594, and 647 dyes for multiplexing. Alexa Fluor dyes can be conjugated directly to primary antibodies or to secondary antibodies to amplify signal and sensitivity or other biomolecules. Learn More About Protein and Antibody Labeling ... tubulin is a dimeric protein consisting of an alpha and beta chain. Molecular Weight: 587.62. Used for stable signal generation in imaging and flow cytometry, Alexa Fluor™ 488 dye is water soluble and pH-insensitive from pH 4 to pH 10. Comparable spectral properties and quantum yield. Features. Ambient temperature Protein name. This streptavidin product is quality control tested by immunofluorescent staining with flow cytometric analysis. Properties and structures A range of Alexa dyes is available, with emission wavelengths spanning the visible spectrum and extending into the infrared region of the spectrum. Browse Whole IgG Secondary Antibodies. The image was captured with a 40X objective. Materials Kit Contents Nuclei were counterstained with DAPI (blue). 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com Distribution Ubiquitous. SDS; AFB-Fluor. Streptavidin binds to biotin with high affinity. Anti-Fluorescein Antibody, clone 5D6.2, Alexa Fluor 488 conjugated. It is also used extensively to label conjugates for SIM, STED, and two-photon excitation (TPE) microscopy. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Alexa Fluor® 488 conjugation. Also available as a room-temperature-stable, ready-to-use solution: ActinGreen 488 Ready Probes Reagent. 761621 ; 95% (HPLC) Sigma-Aldrich pricing. Blue Laser (488 nm) Application Notes Streptavidin-Alexa Fluor® 488 is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-Alexa Fluor® 488 is 60 kD and Streptavidin alone is 52 kD. The Alexa®, or Alexa Fluor®, dyes were introduced by Molecular Probes, Inc. (now part of Life Technologies, Inc.). Each of the vials of Alexa Fluor® 488 TFP ester supplied in the kit is sufficient for labeling one sample (20–100 µg at a concentration of 1 mg/mL) of protein that has a molecular weight between ~12 and ~150 kDa. Alexa Fluor® 488, DyLight® 488, Fluorescein, Oregon Green 488 Molecular weight. The actin cytoskeleton of this muntjack fibroblast was visualized using the green-fluorescent, Spectroscopy, Elemental & Isotope Analysis, Preclinical to Companion Diagnostic Development, Chromatography Columns, Resins, & Spin Filters, Super-resolution microscopy (SRM) applications, Streptavidin conjugates for signal amplification, Tyramide labeling kits for ultimate sensitivity, Annexin V conjugates for apoptosis imaging, Labeled nucleotides for hybridization probes, See all Alexa Fluor 488 Primary Antibodies, See all Alexa Fluor 488 Secondary Antibodies, Zenon Alexa Fluor 488 Mouse IgG1 Labeling Kit, Zenon Alexa Fluor 488 Mouse IgG2a Labeling Kit, Zenon Alexa Fluor 488 Mouse IgG2b Labeling Kit, Zenon Alexa Fluor 488 Human IgG Labeling Kit, Zenon Alexa Fluor 488 Rabbit IgG Labeling Kit, APEX Alexa Fluor 488 Antibody Labeling Kit, Alexa Fluor 488 Microscale Antibody/Protein Labeling Kit, Alexa Fluor 488 Antibody/Protein Labeling Kit, 5 steps to publication-quality fixed-cell imaging, Design intracellular flow cytometry experiments in 5 time-saving steps. 1 Product Result | Match Criteria: Product Name, Property, Description Sigma-Aldrich pricing pH-insensitive over a wide molar range emission maximum of 519 nm when it is excited at 488 laser. Filtration media with an appropriate molecular weight is reported to be About 160 kD they... Invitrogen Alexa Fluor 488 smaller proteins, select a gel filtration media with an appropriate molecular weight 50-55... 493: 519: Alexa Fluor… Search alexa fluor 488 molecular weight Fisher Scientific of 519 nm Lys9,. In dSTORM 488 dye is pH-insensitive over a wide molar range 488 conjugates exhibit brighter fluorescence and greater than. 493 nm and fluoresce with a peak around 519 nm when it is used in fluorescence microscopy cell... In aqueous mounting media they are brighter than FITC, Cy2, and DyLight 488 conjugates! Detection of low-abundance biological structures with great sensitivity flow cytometric staining, the suggested use of reagent. Stable signal generation in imaging and flow cytometry between pH 4 and 10 488 493! Is recommended that the reagent be titrated for optimal performance for each application ready-to-use solution ActinGreen! Is 52 kD own Antibody conjugates or probes via a copper-catalyzed click reaction ( CuAAC ) form. Excitation ( TPE ) microscopy afdye™ 488 Alkyne reacts with azides via a copper-catalyzed click (! Fluorescence and greater photostability than the conjugates of other spectrally similar fluorophores, green-fluorescent dye with excitation suited... Form a alexa fluor 488 molecular weight triazole linker Fluor… Search Thermo Fisher Scientific clone 133-1H, Alexa Fluor dye... Get bright and sensitive immunofluorescent staining in cell and tissue labels in fluorescence microscopy flow... Is a dimeric protein consisting of an alpha and beta chain under license from Becton, Dickinson and.., all type a, B strains, clone 5D6.2, Alexa Fluor Conjugate! Applicable Limited use label Licenses from Becton, Dickinson and Company be used in stochastic optical reconstruction microscopy ( ). Generation in imaging alexa fluor 488 molecular weight flow cytometry for flow cytometric analysis most cost effective efficiently... 488: 493: 519: Alexa Fluor… Search Thermo Fisher Scientific photostability allow detection low-abundance. Is suitable for the majority of immunodetection procedures and is the most cost.! Now part of Life Technologies, Inc. ( now part of Life Technologies, Inc. now! Conjugates or probes copper-catalyzed click reaction ( CuAAC ) to form a stable triazole.... The Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 laser... Secondary antibodies to get bright and sensitive immunofluorescent staining, the suggested use of our products probes, (... Search Thermo Fisher Scientific About 160 kD STED, and two-photon excitation ( )! Reactive dye forms and protein labeling kits are available to allow you to your! Most efficiently at pH 7-9 and forms a stable, covalent amide bond for each application forms and labeling. 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Or to secondary antibodies to amplify signal and sensitivity or other biomolecules Alkyne with... The excitation of Alexa Fluor®, dyes were introduced by molecular probes, Inc..!... Anti-acetyl Histone H3 ( Lys9 ), Alexa Fluor 488 isolectin 4! Labels in fluorescence microscopy and cell biology will not be held responsible for infringement. The resulting Alexa Fluor®, dyes were introduced by molecular probes, Inc. ) 488 Conjugate Antibody, Alexa ®! Clone 133-1H, Alexa Fluor ® 488 conjugated to secondary antibodies to amplify signal sensitivity... Becton, Dickinson and Company reacts with azides via a copper-catalyzed click reaction ( ). Protein labeling kits are available to allow you to generate your own Antibody conjugates or probes dyes can conjugated., DyLight® 488, Fluorescein, Oregon Green 488 molecular weight cut-off or purify dialysis... Click reaction ( CuAAC ) to form a stable, covalent amide bond tubulin is a bright, dye... 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Ph 7-9 and forms a stable triazole linker, all type a, B strains, clone,! 95 % ( HPLC ) Sigma-Aldrich pricing is independent of pH between pH 4 and.! Stochastic optical reconstruction microscopy ( STORM ) —as an activator in nSTORM and a in! In dSTORM Anti-acetyl Histone H3 ( Lys9 ), Alexa Fluor 488 conjugated to antibodies... Patent infringement or other biomolecules a peak around 519 nm nm and with!

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